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Image Search Results
Journal: Clinical cancer research : an official journal of the American Association for Cancer Research
Article Title: Krüppel-like Factor 4 Suppresses Serine/Threonine Kinase 33 Activation and Metastasis of Gastric Cancer through Reversing Epithelial–Mesenchymal Transition
doi: 10.1158/1078-0432.CCR-17-3346
Figure Lengend Snippet: A, left: shown are phase contrast microphotographs of BCG-823 cells infected with either empty vector or STK33-overexpressing vector and MGC-803 transfected with either non-targeting siRNA (siNC) or siSTK33. Higher magnification of the highlighted region is shown in the inset. Right: expression of E-cadherin and vimentin detected by immunofluorescence. Nuclear counterstaining with diamidino-2-phenylindole (DAPI) is shown separately. Scale bars: 50 μm. B, expression of STK33, E-cadherin and Vimentin detected by western blotting, in total lysates from BCG-823 and MGC-803 cells infected with the indicated constructs. β-actin was used as a loading control.
Article Snippet: Cells were mounted with 4’,6-diamidino-2-phenylindole (DAPI) Fluoromount-G medium and a
Techniques: Infection, Plasmid Preparation, Transfection, Expressing, Immunofluorescence, Western Blot, Construct